Proc Natl Acad Sci U S A. auth.: group Franken

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Proc Natl Acad Sci U S A. 2021 Jun 22;118(25):e2017364118. doi: 10.1073/pnas.2017364118.

Dissecting and modeling photic and melanopsin effects to predict sleep disturbances induced by irregular light exposure in mice

Jeffrey Hubbard 1 2, Mio Kobayashi Frisk 1 2, Elisabeth Ruppert 1 2, Jessica W Tsai 3, Fanny Fuchs 1 2, Ludivine Robin-Choteau 1 4, Jana Husse 5, Laurent Calvel 1 2, Gregor Eichele 5, Paul Franken 6, Patrice Bourgin 7 2

Abstract

Artificial lighting, day-length changes, shift work, and transmeridian travel all lead to sleep-wake disturbances. The nychthemeral sleep-wake cycle (SWc) is known to be controlled by output from the central circadian clock in the suprachiasmatic nuclei (SCN), which is entrained to the light-dark cycle. Additionally, via intrinsically photosensitive retinal ganglion cells containing the photopigment melanopsin (Opn4), short-term light-dark alternations exert direct and acute influences on sleep and waking. However, the extent to which longer exposures typically experienced across the 24-h day exert such an effect has never been clarified or quantified, as disentangling sustained direct light effects (SDLE) from circadian effects is difficult. Recording sleep in mice lacking a circadian pacemaker, either through transgenesis (Syt10 cre/cre Bmal1 fl/- ) or SCN lesioning and/or melanopsin-based phototransduction (Opn4 -/- ), we uncovered, contrary to prevailing assumptions, that the contribution of SDLE is as important as circadian-driven input in determining SWc amplitude. Specifically, SDLE were primarily mediated (>80%) through melanopsin, of which half were then relayed through the SCN, revealing an ancillary purpose for this structure, independent of its clock function in organizing SWc. Based on these findings, we designed a model to estimate the effect of atypical light-dark cycles on SWc. This model predicted SWc amplitude in mice exposed to simulated transequatorial or transmeridian paradigms. Taken together, we demonstrate this SDLE is a crucial mechanism influencing behavior on par with the circadian system. In a broader context, these findings mandate considering SDLE, in addition to circadian drive, for coping with health consequences of atypical light exposure in our society.

Keywords: circadian and noncircadian; melanopsin; photoperiods; phototransduction; sleep–wake cycle.