Genotyping facility : CIG common facility
Mission
The main activity of this service is to screen (by specific PCR amplification) transgenic mice housed at the CIG animal facility.
The staff
| Head of the facility: | Fabienne Lammers (Monday and Wednesday all day, Tuesday and Friday AM) Fabienne.Lammers (at) unil.ch |
| Lab technicians : | Katharina Hausherr (Tuesday, Wednesday, Thursday) Katharina.Hausherr (at) unil.ch |
| Supervisor : | Nicole Vouilloz Nicole.Vouilloz (at) unil.ch |
| Scientific advisor : | Professor Alexandre Reymond Alexandre.Reymond (at) unil.ch |
The facility is located in laboratory 2013 in the Génopode building (tel : 021/692 4136).
For any request please contact : cig_genotyping (at) unil.ch
Genotyping
| The facility realises routine PCR with the use of a robot (Freedom EVO from Tecan) for series of 20 or more samples. |  |
The robot performs pipeting manipulations for :
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Screens made in routine are available on request.
The facility is able to transfer manual screen to robot analyse (primers testing, DNA extraction, amplification parameters…) or to optimize a new screen (for example moving from Southern to PCR analysis, establishment of a new protocol…).
Results of the genotyping are entered by the facility into the Pyrat system database, co-managed with the animal facility.
An analyses request form should be joined with the samples. Links to the request form (en/fr):
Samples are stored 6 months in the laboratory after genotyping.
Price of analyses are fixed by the CIG direction and bills are established for each research group.
For any request please contact : cig_genotyping (at) unil.ch