DNA Sequencing Service
Mission
The mission of the CIG DNA sequencing facility includes the sequencing of PCR products or plasmids, the sizing and relative quantification of fluorescence labelled DNA fragments and the advising and troubleshooting on sequencing and fragment analysis projects.
Activities
- DNA sequencing service for CIG’s groups
- DNA sequencing services for the Biophore’s groups
- DNA fragments analysis on request (microsatellites, T-RFPL)
- management of Big Dye stocks
- purification of sequencing samples
- advice and collaboration with all the users of the service
Available equipement
- Applied Biosystems 3730 DNA Analyser equipped with an array of forty eight 50cm capillaries ( room 3025, 3rd floor Genopode Building)
- Purification columns in EDGE 96 well plate format
3730 DNA Analyser exterior
3730 DNA Analyser interior
CIG Sequencing Service Guidelines
Long sequencing reads
Typical read lengths are in the range of 750 to 900 bases and may reach 1000 bases for optimal template and primers.
For some samples shorter read length should be expected e.g. for templates with secondary structures, stretches of A and T, GC-rich regions or large sizes.
Figure with a sample in the Electropherogram view |
Software
- Applied Biosystems DNA Sequencing Analysis software version 5.2
- GeneMapper software version 4.0
Contact
| Technician responsible for the service |
Marina Pronina (Tuesday 14:00 – 18:00, Wednesday and Thursday: 8:30 – 12:30, and Friday 8:30 – 17:30) |
Phone 3992 or 3959 |
| Technician | Gilles Boss (Monday and Tuesday morning) |
Phone 3959 or 3952 |
| Supervisor | Keith Harshman | Phone 3906 |