Genome-wide re-sequencing of multi-drug resistant Mycobacterium leprae Airaku-3.
Abstract
Genotyping and molecular characterization of drug resistance mechanisms in Mycobacterium leprae enables disease transmission and drug resistance trends to be monitored. In the present study, we performed genome-wide analysis of Airaku-3, a multidrug resistant strain with an unknown mechanism of resistance to rifampicin. We identified 12 unique non-synonymous single-nucleotide polymorphisms (SNPs) including two in the transporter-encoding ctpC and ctpI genes. In addition, two SNPs were found that improve the resolution of SNP-based genotyping, particularly for Venezuelan and South-East Asian strains of M. leprae. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Mycobacterium leprae , Rifampicin-resistance, Single Nucleotide Polymorphism (SNP), leprosy, molecular epidemiology
- PMID:
- 24612452