Curr Protoc Mouse Biol.: auth.: group Reymond

Curr Protoc Mouse Biol. 2016 Sep 1;6(3):307-332. doi: 10.1002/cpmo.12.

Histomorphological Phenotyping of the Adult Mouse Brain.

Mikhaleva A1, Kannan M1,2,3,4,5, Wagner C2,3,4,5, Yalcin B1,2,3,4,5.

Author information

  • 1Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.
  • 2Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.
  • 3National Centre for Scientific Research, UMR7104, Illkirch, France.
  • 4National Institute of Health and Medical Research, U964, Illkirch, France.
  • 5University of Strasbourg, Illkirch, France.


This article describes a series of standard operating procedures for morphological phenotyping of the mouse brain using basic histology. Many histological studies of the mouse brain use qualitative approaches based on what the human eye can detect. Consequently, some phenotypic information may be missed. Here we describe a quantitative approach for the assessment of brain morphology that is simple and robust. A total of 78 measurements are made throughout the brain at specific and well-defined regions, including the cortex, the hippocampus, and the cerebellum. Experimental design and timeline considerations, including strain background effects, the importance of sectioning quality, measurement variability, and efforts to correct human errors are discussed. © 2016 by John Wiley & Sons, Inc.

Copyright © 2016 John Wiley & Sons, Inc.


brain morphology; histology; mouse; phenotyping

PMID: 27584555