Postdoctoral position in CTG Repeat Instability and Myotonic Dystrophy, Imagine Institute, Paris (FR)

Post-­doctoral Position

Laboratory of CTG Repeat Instability and Myotonic Dystrophy • Imagine Institute
Paris, France

RNA toxicity and neuroglial miscommunication in myotonic dystrophy brains

A  two‐year  postdoctoral  position  is  available  from  February  2018,  in  the  Gourdon  laboratory  of  CTG  Repeat Instability and Myotonic Dystrophy at the new Imagine Institute for Genetic Diseases (,  in  Paris,  France.  The  project  is  funded  by  the  French  “Agence  Nationale de Recherche” (ANR), and coordinated by Geneviève Gourdon and Mario Gomes-­Pereira.

Project summary
The  central  nervous  system  (CNS)  consists  in  a  complex  mixture  of  interacting  cell  types,  each  with  differing contributions to neurological disease. In myotonic dystrophy type 1 (DM1), expanded non-‐coding trinucleotide DNA repeats are transcribed into toxic RNA in multiple CNS cell types, and patients experience learning/memory deficits, cognitive/executive dysfunction, profound fatigue and excessive sleepiness.  While  DM1  is  established  to  result  from  sequestration  and  dysregulation  of  multiple  RNA-­binding proteins, a major gap exists in our understanding of how molecular events are connected to neurological  symptoms,  and  the  extent  to  which  individual  brain  cell  types  drive  pathology.  We  have  previously gathered evidence of neuronal and glial dysfunction in DM1 brains. Now, taking advantage of mouse and human pluripotent stem cell-­derived models, we will investigate brain mechanisms of RNA toxicity  with  cell  type  resolution.  The  combination  of  global  molecular  methods  of  deep  RNA  sequencing and proteomics, with electrophysiological recordings and high-­resolution microscopy will help us understand how the DM1 trinucleotide repeat expansion perturbs the function of specific CNS cell types and affects their critical interplay.

Candidates  must  hold  a  PhD  in  a  relevant  subject  for  the  project  (molecular  biology,  cell  biology,  neuroscience or genetics). They must be trained in molecular and cell biology. Knowledge of neurobiology is desirable. The successful candidate is expected to be highly motivated, well-­organised, have  excellent  communication  skills,  a  high  level  of  accuracy  in  all  aspects  of  work  and  the  ability  to  contribute to a multidisciplinary project and research group with insight and enthusiasm. He/she will (1) provide  input  into  decisions  on  experimental  approaches  and  techniques;  (2)  keep  up  to  date  with  current developments in the field; (3) collaborate with other members of the group and liaise with external collaborators; (4) take a lead in the presentation of results and paper writing. Good knowledge of the English language is essential.

Salary will be commensurate with experience following Inserm guidelines. 

Application details
Applications in English should include a cover letter, a detailed CV and the contact details of at least two academic  references.  Applications  and  informal  inquiries  should  be  e-­mailed  to  Mario  Gomes-­Pereira  ( Please send your application before 31st October 2017. Imagine – Institut des maladies génétiques
24 boulevard du Montparnasse -­ 75015 PARIS France