Mol Biol Cell. 2010 Dec 9. [Epub ahead of print]
Actin cables and the exocyst form two independent morphogenesis pathways in the fission yeast.
Bendezú FO, Martin SG.
Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Genopode building, CH-1015 Lausanne, Switzerland.
Cell morphogenesis depends on polarized exocytosis. One widely held model posits that long-range transport and exocyst-dependent tethering of exocytic vesicles at the plasma membrane sequentially drive this process. Here, we describe that disruption of either actin-based long-range transport and microtubules or the exocyst did not abolish polarized growth in rod-shaped fission yeast cells. However, disruption of both actin cables and exocyst led to isotropic growth. Exocytic vesicles localized to cell tips in single mutants but were dispersed in double mutants. In contrast, a marker for active Cdc42, a major polarity landmark, localized to discreet cortical sites even in double mutants. Localization and photobleaching studies show that the exocyst subunits Sec6 and Sec8 localize to cell tips largely independently of the actin cytoskeleton, but in a cdc42 and phospholipid phosphatidylinositol 4,5-bisphosphate (PIP(2))-dependent manner. Thus, in fission yeast, long-range cytoskeletal transport and PIP(2)-dependent exocyst represent parallel morphogenetic modules downstream of Cdc42, raising the possibility of similar mechanisms in other cell types.
PMID: 21148300 [PubMed – as supplied by publisher]
“L’Uniscope” no 559 (6.12.2010 – 31.1.2011) :
Les uns les autres
Special seminar (EMBO LTF applicant)
University of Edinburgh
“Maintenance of Telomeres and Centromeres in Fission Yeast”
Thursday November 4th 2010
All the interested people are cordially invited.
Host : Prof. Sophie Martin
Sophie Martin got a ERC (European Research Council) Starting Independent Researcher Grant in July 2010.
L’European Research Council (ERC) vient de faire connaître les lauréats des «Starting Grants 2010», bourses de jeunes chercheurs indépendants. Un montant de 580 millions d’euros a été octroyé à 427 scientifiques talentueux issus de 39 nationalités. Parmi eux, Frédéric Gachon et Sophie Martin de la Faculté de biologie et de médecine de l’UNIL. (See the whole text).
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Changins meeting : November 11, 2010.
This meeting aims at increasing the exchanges between researchers of the Lausanne-Geneva region and is open to all (registration is free, but you must register to attend). This year’s topic is: “Aging and Longevity”, but poster presentations will include the entire research fields of the Lausanne-Geneva region.
More information and for registration
Manasi Kelkar obtained her Master in Zoology with specialization in Molecular Biology in the Department of Zoology of the University of Pune (India). During her studentship she worked on the project : “Differential expression patterns of DNA methyltransferases in mice tissues”.
She will join Sophie Martin’s lab as a PhD Student on October 1st, 2010.