Monalisa Das obtained her Master’s degree in microbiology from Allahabad Agricultural Institute, India, and joined Prof. M. R. S Rao’s Lab at JNCASR, Bangalore, where she worked on the role of non-coding RNAs in cancer. Subsequently, she moved to Switzerland and worked with Prof. Rory Johnson at the University of Bern, where she studied the role of transposable elements in the localization of long noncoding RNAs.
She joined Prof. Van Leeuwen’s lab in January 2021 for few months to work on the project involving the generation of knockout human cell lines to screen for genetic suppression interactions for DNA damage repair genes.
Dr. Leopold Parts
Wellcome Trust Sanger Institute, UK
“Scaling up CRISPR/Cas screens to measure natural variation in human gene essentiality”
Genetic screens based on CRISPR/Cas technology are a powerful tool for understanding cellular phenotypes. However, the coverage and replicate requirements result in large experiment sizes, which are limiting when samples are scarce, or the protocols are expensive and laborious. I will discuss our attempts to reduce the scale of genome-wide screens, with the aim of rendering hundreds of them achievable in human induced pluripotent stem cells. To do so, we measure and predict Cas9 editing outcomes, build a statistical model of noise and information sharing across many CRISPR/Cas experiments, and design a reduced gRNA library that allows evaluating two edits in each cell. I will also present preliminary findings from using these advances to measure the variation in gene essentiality in cells from tens of healthy donors.
Tuesday February 4th 2020
All the interested people are cordially invited.
Host : Prof. Jolanda van Leeuwen