Uncategorized Archive

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Prof. J. van Leeuwen was interviewed for the RTS radio program “CQFD” on May 10, 2022 about CRISPR

Listen to the short program: https://pages.rts.ch/la-1ere/programmes/cqfd/13046064-comment-crispr-le-couteau-suisse-de-la-genetique-ne-cesse-detre-ameliore-10-05-2022.html?mediaShare=1

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Life Sci Alliance.: auth.: group van Leeuwen

Life Sci Alliance. 2022 Jan 11;5(4):e202101153. doi: 10.26508/lsa.202101153. Print 2022 Apr.

Chl1 helicase controls replication fork progression by regulating dNTP pools

Amandine Batté 1Sophie C van der Horst 1Mireille Tittel-Elmer 1 2Su Ming Sun 1Sushma Sharma 3Jolanda van Leeuwen 4Andrei Chabes 3Haico van Attikum 5

Abstract

Eukaryotic cells have evolved a replication stress response that helps to overcome stalled/collapsed replication forks and ensure proper DNA replication. The replication checkpoint protein Mrc1 plays important roles in these processes, although its functional interactions are not fully understood. Here, we show that MRC1 negatively interacts with CHL1, which encodes the helicase protein Chl1, suggesting distinct roles for these factors during the replication stress response. Indeed, whereas Mrc1 is known to facilitate the restart of stalled replication forks, we uncovered that Chl1 controls replication fork rate under replication stress conditions. Chl1 loss leads to increased RNR1 gene expression and dNTP levels at the onset of S phase likely without activating the DNA damage response. This in turn impairs the formation of RPA-coated ssDNA and subsequent checkpoint activation. Thus, the Chl1 helicase affects RPA-dependent checkpoint activation in response to replication fork arrest by ensuring proper intracellular dNTP levels, thereby controlling replication fork progression under replication stress conditions.

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I. Lopez-Mejia and N. Vastenhouw were in “L’Actu” magazine regarding their FBM interdisciplinary Grant, Dec. 2021

Among the 4 interdisciplinary projects selected by the Faculty of Biology and Medicine to get fundings, 2 were proposed by members of the CIG:

List of all awardees on the UNIL web magazine: https://news.unil.ch/display/1638977433273

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Congratulations to several Genopode (ex-)members on their participation in the Vortex race of 3km run!

On 43 teams running the Vortex race last Nov. 25th, the Génopode’s ones arrived 11th and 24th

Congratulation to the participants!

11th: Team les mardistes composed of Carlos L., Manuel B. and Gérard B.
24th: Team Jeyaly composed of Yann E., Lyam B. and Jean-Yves R.

All results can be found here: https://4bd5409b-b6db-46f8-b130-1f5610ea87d6.filesusr.com/ugd/bc83a2_e8dfd4e052fb4a07b2693932fad98ff4.pdf

Further information on the run: https://www.essul.ch/

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Open position as PhD student in Neurodevelopmental biology at the CIG, UNIL (CH)

>>>Link to the job add<<<

Introduction

UNIL is a leading international teaching and research institution, with over 5,000 employees and 17,000 students split between its Dorigny campus, CHUV and Epalinges. As an employer, UNIL encourages excellence, individual recognition and responsibility.

Presentation

The Center for Integrative Genomics of the University of Lausanne invites applications for a PhD Student position in the research group of Dr Sophie Croizier. The Croizier lab works on the role of guidance molecules in the formation of hypothalamic circuits controlling energy and glucose homeostasis. For more details, please consult the current website of the group: https://unil.ch/cig/home/menuinst/research/dr-sophie-croizier.html

Job information

Expected start date: 01.01.2022 or to be discussed

Contract length: 1 year, renewable, maximum 3 years

Activity rate: 85%

Workplace: University of Lausanne, Center for Integrative Genomics (CIG): https://www.unil.ch/cig/home.html

Your responsibilities

The successful candidate will work on the lab project to untangle the molecular mechanisms underlying the development of the sexually dimorphic stress-related feeding-circuits This work will rely on multidisciplinary approaches including molecular biology, 3D cultures, histology and microscopy/image analysis, stereotaxic surgeries, and mouse behavior/physiology.

Contact for further information

Dr Sophie Croizier, PhD

e-mail: sophie.croizier@unil.ch

Your qualifications

In order to complete our team, we are looking for someone with the following skills:

  • Experience in histological approaches
  • Experience in mouse physiology (food intake)
  • Dynamic, and enthusiastic student with a master thesis in life science, interested in working in a highly interactive environment.
  • Excellent command of oral and written English

What the position offers you

We offer a nice working place in a multicultural, diverse and dynamic academic environment. Opportunities for professional training, a lot of activities and other benefits to discover.

Your application

Deadline : 07.11.2021

Please, send your full application in Word or PDF.
Only applications through this website will be taken into account.

We thank you for your understanding.

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Int J Mol Sci.: co-auth.: W.Wahli

Int J Mol Sci. 2021 Aug 9;22(16):8555. doi: 10.3390/ijms22168555.

The PPARβ/δ-AMPK Connection in the Treatment of Insulin Resistance

David Aguilar-Recarte 1 2 3Xavier Palomer 1 2 3Walter Wahli 4 5 6Manuel Vázquez-Carrera 1 2 3

Abstract

The current treatment options for type 2 diabetes mellitus do not adequately control the disease in many patients. Consequently, there is a need for new drugs to prevent and treat type 2 diabetes mellitus. Among the new potential pharmacological strategies, activators of peroxisome proliferator-activated receptor (PPAR)β/δ show promise. Remarkably, most of the antidiabetic effects of PPARβ/δ agonists involve AMP-activated protein kinase (AMPK) activation. This review summarizes the recent mechanistic insights into the antidiabetic effects of the PPARβ/δ-AMPK pathway, including the upregulation of glucose uptake, muscle remodeling, enhanced fatty acid oxidation, and autophagy, as well as the inhibition of endoplasmic reticulum stress and inflammation. A better understanding of the mechanisms underlying the effects resulting from the PPARβ/δ-AMPK pathway may provide the basis for the development of new therapies in the prevention and treatment of insulin resistance and type 2 diabetes mellitus.

Keywords: AMPK; GDF15; PPARβ/δ; insulin resistance; type 2 diabetes mellitus.