Please, find below the closing and re-opening dates of the following central services:

Stock, maintenance and ordering: closes from Friday, Dec. 23, at 11:15 AM and opens again Monday, Jan. 3 morning.
Washing facility:  closes from Friday, Dec. 23, at 15:30 and opens again Monday, Jan. 3 morning.
Central administration: closes from Friday, Dec. 23, at 11:30 and opens again Monday, Jan. 3 morning.
IT support: closes from Friday, Dec. 23, at 17:00 and opens again Monday, Jan. 3 morning.
If you need computer support within of these dates, please contact the helpdesk of Unil by phone at 22.11 or by e-mail at helpdesk (at) unil.ch

Scientific Services of the CIG:

Phenotyping: closes from Friday, Dec. 23, at 12:00 PM and opens again Monday, Jan. 3 morning.
Genotyping: closes from Friday, Dec. 23, at 15:00 and opens again Tuesday, Jan. 8 morning.
Cell culture and solutions: closes from Friday, Dec. 16, at 16:00 and opens again Monday, Jan. 3 morning.
Biofluid assays, Workshop, General technical support: closes from Friday, Dec. 23, at 16:00 and opens again Monday, Jan. 3 morning.
Metabolic analysis in Mice: closes from Friday, Dec. 23, at 16:00 and opens again Monday, Jan. 3 morning.

Have a nice holiday!

J Clin Invest. 2022 Jul 1;132(13):e162359. doi: 10.1172/JCI162359.

CDK4 is an essential insulin effector in adipocytes

Sylviane Lagarrigue, Isabel C Lopez-Mejia, Pierre-Damien Denechaud, Xavier Escoté, Judit Castillo-Armengol, Veronica Jimenez, Carine Chavey, Albert Giralt, Qiuwen Lai, Lianjun Zhang, Laia Martinez-Carreres, Brigitte Delacuisine, Jean-Sébastien Annicotte, Emilie Blanchet, Sébastien Huré, Anna Abella, Francisco J Tinahones, Joan Vendrell, Pierre Dubus, Fatima Bosch, C Ronald Kahn, Lluis Fajas

• PMID: 35775487
• PMCID: PMC9246374
• DOI: 10.1172/JCI162359

Cells. 2022 May 25;11(11):1737. doi: 10.3390/cells11111737.

Homocysteine Metabolism Pathway Is Involved in the Control of Glucose Homeostasis: A Cystathionine Beta Synthase Deficiency Study in Mouse

Céline Cruciani-Guglielmacci ¹, Kelly Meneyrol ¹, Jessica Denom ¹, Nadim Kassis ¹, Latif Rachdi ², Fatna Makaci ², Stéphanie Migrenne-Li ¹, Fabrice Daubigney ¹, Eleni Georgiadou ³, Raphaël G Denis ^1 2, Ana Rodriguez Sanchez-Archidona ⁴, Jean-Louis Paul ⁵, Bernard Thorens ⁴, Guy A Rutter ^3 6 7, Christophe Magnan ¹, Hervé Le Stunff ⁸, Nathalie Janel ¹

Abstract

Cystathionine beta synthase (CBS) catalyzes the first step of the transsulfuration pathway from homocysteine to cystathionine, and its deficiency leads to hyperhomocysteinemia (HHcy) in humans and rodents. To date, scarce information is available about the HHcy effect on insulin secretion, and the link between CBS activity and the setting of type 2 diabetes is still unknown. We aimed to decipher the consequences of an inborn defect in CBS on glucose homeostasis in mice. We used a mouse model heterozygous for CBS (CBS+/-) that presented a mild HHcy. Other groups were supplemented with methionine in drinking water to increase the mild to intermediate HHcy, and were submitted to a high-fat diet (HFD). We measured the food intake, body weight gain, body composition, glucose homeostasis, plasma homocysteine level, and CBS activity. We evidenced a defect in the stimulated insulin secretion in CBS+/- mice with mild and intermediate HHcy, while mice with intermediate HHcy under HFD presented an improvement in insulin sensitivity that compensated for the decreased insulin secretion and permitted them to maintain a glucose tolerance similar to the CBS+/+ mice. Islets isolated from CBS+/- mice maintained their ability to respond to the elevated glucose levels, and we showed that a lower parasympathetic tone could, at least in part, be responsible for the insulin secretion defect. Our results emphasize the important role of Hcy metabolic enzymes in insulin secretion and overall glucose homeostasis.

Keywords: autonomic nervous system; hyperhomocysteinemia; insulin secretion; type 2 diabetes.

• PMID: 35681432
• PMCID: PMC9179272
• DOI: 10.3390/cells11111737