EMBO Rep.; auth.: D.Gatfield

EMBO Rep. 2012 Oct 19. doi: 10.1038/embor.2012.158. [Epub ahead of print]

CAVIN-3 regulates circadian period length and PER:CRY protein abundance and interactions.


Department of Molecular Biology, Sciences III, University of Geneva, Geneva 1211, Switzerland.


In mammals, transcriptional autorepression by Period (PER) and Cryptochrome (CRY) protein complexes is essential for the generation of circadian rhythms. We have identified CAVIN-3 as a new, cytoplasmic PER2-interacting protein influencing circadian clock properties. Thus, CAVIN-3 loss- and gain-of-function shortened and lengthened, respectively, the circadian period in fibroblasts and affected PER:CRY protein abundance and interaction. While depletion of protein kinase Cδ (PKCδ), a known partner of CAVIN-3, had little effect on circadian gene expression, CAVIN-3 required the PKCδ-binding site to exert its effect on period length. This suggests the involvement of yet uncharacterized protein kinases. Finally, CAVIN-3 activity in circadian gene expression was independent of caveolae.





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