Plant Cell.; co-author group Fankhauser

Plant Cell. 2013 Feb 1. [Epub ahead of print]

Phosphorylation of Phytochrome B Inhibits Light-Induced 

Signaling via Accelerated DarkReversion in Arabidopsis.

Source

Faculty of Biology, University of Freiburg, D-79104, Freiburg Germany.

Abstract

The photoreceptor phytochrome B (phyB) interconverts between the biologically active Pfr (λ(max) = 730 nm) and inactive Pr (λ(max) = 660 nm) forms in a red/far-red-dependent fashion and regulates, as molecular switch, many aspects of light-dependent development in Arabidopsis thaliana. phyB signaling is launched by the biologically active Pfr conformer and mediated by specific protein-protein interactions between phyB Pfr and its downstream regulatory partners, whereas conversion of Pfr to Pr terminates signaling. Here, we provide evidence that phyB is phosphorylated in planta at Ser-86 located in the N-terminal domain of the photoreceptor. Analysis of phyB-9 transgenic plants expressing phospho-mimic and nonphosphorylatable phyB-yellow fluorescent protein (YFP) fusions demonstrated that phosphorylation of Ser-86 negatively regulates all physiological responses tested. The Ser86Asp and Ser86Ala substitutions do not affect stability, photoconversion, and spectral properties of the photoreceptor, but light-independent relaxation of the phyB(Ser86Asp) Pfr into Pr, also termed dark reversion, is strongly enhanced both in vivo and in vitro. Faster dark reversion attenuates red light-induced nuclear import and interaction of phyB(Ser86Asp)-YFP Pfr with the negative regulator PHYTOCHROMEINTERACTING FACTOR3 compared with phyB-green fluorescent protein. These data suggest that accelerated inactivation of the photoreceptor phyB via phosphorylation of Ser-86 represents a new paradigm for modulating phytochrome-controlledsignaling.

PMID:

 23378619

[PubMed – as supplied by publisher]