PLoS One; auth.: group Benton

PLoS One. 2012;7(11):e48381. doi: 10.1371/journal.pone.0048381. Epub 2012 Nov 7.

Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila.


Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland ; Laboratory of Intelligent Systems, Institute of Microengineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.


Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila.