Proteomics; co-auth: PAF

Proteomics. 2011 Sep 14. doi: 10.1002/pmic.201100234. [Epub ahead of print]

Identification of novel secreted proteases during extracellular proteolysis by dermatophytes at acidic pH.


Department of Dermatology, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland.


The dermatophytes are a group of closely related fungi which are responsible for the great majority of superficial mycoses in humans and animals. Among various potential virulence factors, their secreted proteolytic activity attracts a lot of attention. Most dermatophyte secreted proteases which have so far been isolated in vitro are neutral or alkaline enzymes. However, inspection of the recently decoded dermatophyte genomes revealed many other hypothetical secreted proteases, in particular acidic proteases similar to those characterized in Aspergillus spp. The validation of such genome predictions instigated the present study on two dermatophyte species, Microsporum canis and Arthroderma benhamiae. Both fungi were found to grow well in a protein medium at acidic pH, accompanied by extracellular proteolysis. Shotgun mass spectrometry analysis of secreted protein revealed fundamentally different protease profiles during fungal growth in acidic versus neutral pH conditions. Most notably, novel dermatophyte secreted proteases were identified at acidic pH such as pepsins, sedolisins and acidic carboxypeptidases. Therefore, our results not only support genome predictions, but demonstrate for the first time the secretion of acidic proteases by dermatophytes. Our findings also suggest the existence of different pathways of protein degradation into amino acids and short peptides in these highly specialized pathogenic fungi.

Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.





[PubMed – as supplied by publisher]

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