Methods Mol Biol.: auth.: group Fankhauser

Methods Mol Biol. 2021;2297:21-31. doi: 10.1007/978-1-0716-1370-2_3.

Analysis of Shade-Induced Hypocotyl Elongation in Arabidopsis

Yetkin Çaka Ince¹, Vinicius Costa Galvão²Affiliations expand

Abstract

The presence of neighbor or overtopping plants is perceived by changes in light quality, which lead to several growth and developmental changes known as shade avoidance syndrome (SAS). Among them, the analysis of hypocotyl elongation is an important SAS physiological output that has been successfully used to investigate photoreceptors and downstream signaling components. Here we describe the experimental setup and growth conditions used to investigate photoreceptors and their signaling mechanisms through the analysis of hypocotyl elongation in laboratory, using simulated low R/FR ratio, low blue light, and true/deep shade conditions.

Keywords: Hypocotyl; Low R/FR ratio; Low blue; Shade avoidance; True shade.

PMID: 33656666
DOI: 10.1007/978-1-0716-1370-2_3