Recent publications
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PLoS Genet.: co-auth.: F.Schütz

 2019 Feb 8;15(2):e1007905. doi: 10.1371/journal.pgen.1007905. [Epub ahead of print]

Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment.

Abstract

RNA interference (RNAi) related pathways are essential for germline development and fertility in metazoa and can contribute to inter- and trans-generational inheritance. In the nematode Caenorhabditis elegans, environmental double-stranded RNA provided by feeding can lead to heritable changes in phenotype and gene expression. Notably, transmission efficiency differs between the male and female germline, yet the underlying mechanisms remain elusive. Here we use high-throughput sequencing of dissected gonads to quantify sex-specific endogenous piRNAs, miRNAs and siRNAs in the C. elegans germline and the somatic gonad. We identify genes with exceptionally high levels of secondary 22G RNAs that are associated with low mRNA expression, a signature compatible with silencing. We further demonstrate that contrary to the hermaphrodite germline, the male germline, but not male soma, is resistant to environmental RNAi triggers provided by feeding, in line with previous work. This sex-difference in silencing efficacy is associated with lower levels of gonadal RNAi amplification products. Moreover, this tissue- and sex-specific RNAi resistance is regulated by the germline, since mutant males with a feminized germline are RNAi sensitive. This study provides important sex- and tissue-specific expression data of miRNA, piRNA and siRNA as well as mechanistic insights into sex-differences of gene regulation in response to environmental cues.

PMID: 30735500

 

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CIG Snow Day 2019

Thanks to all the participants at the CIG Snow Day 2019 (Sledge – Snowshoeing – Ski)

  

  

   

 

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Nat Commun.: auth.: group Benton

 2019 Feb 7;10(1):643. doi: 10.1038/s41467-019-08345-4.

Sensory neuron lineage mapping and manipulation in the Drosophila olfactory system.

Abstract

Nervous systems exhibit myriad cell types, but understanding how this diversity arises is hampered by the difficulty to visualize and genetically-probe specific lineages, especially at early developmental stages prior to expression of unique molecular markers. Here, we use a genetic immortalization method to analyze the development of sensory neuron lineages in the Drosophila olfactory system, from their origin to terminal differentiation. We apply this approach to define a fate map of nearly all olfactory lineages and refine the model of temporal patterns of lineage divisions. Taking advantage of a selective marker for the lineage that gives rise to Or67d pheromone-sensing neurons and a genome-wide transcription factor RNAi screen, we identify the spatial and temporal requirements for Pointed, an ETS family member, in this developmental pathway. Transcriptomic analysis of wild-type and Pointed-depleted olfactory tissue reveals a universal requirement for this factor as a switch-like determinant of fates in these sensory lineages.

PMID: 30733440

 

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Welcome Antoine!

My name is Antoine Rohrbach. I have just joined Prof Bernard Thorens’ group as a lab technician and I will work with Fanny Langlet. I got my CFC last year in the lab of Prof Ivan Rodriguez at the Neurobiology Department of the UNIGE.

 

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Welcome to Céline!

Hello, my name is Céline Lukowicz and I have joined Prof. Liliane Michalik’s lab as a postdoctoral researcher in February 2019.

After graduating in biology and pharmacological innovation, I worked in Dr. Hervé Guillou’s team during my PhD in Integrative Toxicology and Metabolism at the Institute for Agricultural Research, University of Toulouse (France) till July 2018.

My PhD research project focused on the sexually dimorphic role of the nuclear receptor CAR (Constitutive Androstane Receptor) in energy homeostasis and xenobiotic detoxication. I investigated the metabolic role of the nuclear receptor CAR and the long-term effect of a chronic dietary exposure to pesticides in vivo, with a special emphasis on the role of CAR on the sexually dimorphic deleterious effects of this pesticide cocktail.

At the CIG, my postdoctoral project is to investigate the mechanism underlying the initiation of a pro-tumorigenic cascade in response to UV insults in skin cells.


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Oncotarget.: auth.: group Michalik

 2018 Dec 28;9(102):37614-37615. doi: 10.18632/oncotarget.26532. eCollection 2018 Dec 28.

The Janus face of rosiglitazone.

KEYWORDS:

PPARγ; melanoma; rosiglitazone; tumor microenvironment

PMID: 30701018