Recent publications
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Acta Physiol (Oxf).: co-auth.: GTF

 2020 Feb 18:e13457. doi: 10.1111/apha.13457. [Epub ahead of print]

Renal tubular Arginase-2 participates in the formation of the corticomedullary urea gradient and attenuates kidney damage in ischemia-reperfusion injury in mice.

Abstract

AIM:

Arginase 2 (ARG2) is a mitochondrial enzyme that catalyzes hydrolysis of L-arginine into urea and L-ornithine. In the kidney, ARG2 is localized to the S3 segment of the proximal tubule. It has been shown that expression and activity of this enzyme are upregulated in a variety of renal pathologies, including ischemia-reperfusion (IR) injury. However, the (patho)physiological role of ARG2 in the renal tubule remains largely unknown.

METHODS:

We addressed this question in mice with conditional knockout of Arg2 in renal tubular cells (Arg2lox/lox /Pax8-rtTA/LC1 or, cKO mice).

RESULTS:

We demonstrate that cKO mice exhibit impaired urea concentration and osmolality gradients along the corticomedullary axis. In a model of unilateral IR injury (UIRI) with an intact contralateral kidney, ischemia followed by 24 hours of reperfusion resulted in significantly more pronounced histological damage in ischemic kidneys from cKO mice compared to control and sham-operated mice. In parallel, UIRI-subjected cKO mice exhibited a broad range of renal functional abnormalities, including albuminuria and aminoaciduria. Fourteen days after UIRI, the cKO mice exhibited complex phenotype characterized by significantly lower body weight, increased plasma levels of early predictive markers of kidney disease progression (ADMA and SDMA), impaired mitochondrial function in the ischemic kidney but no difference in kidney fibrosis as compared to control mice.

CONCLUSION:

Collectively, these results establish the role of ARG2 in the formation of corticomedullary urea and osmolality gradients and suggest that this enzyme attenuates kidney damage in ischemia-reperfusion injury.

KEYWORDS:

arginase-2; arginine; kidney; kidney injury; proximal tubule; urea

PMID: 32072766

 

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Thesis defense of Pasquelena De Nittis, Friday Feb. 28, 2020 – 3:00 pm in Aud. B, Génopode

Elena De Nittis  will present her public thesis defense entitled: “Genetics of IDDCA syndrome”.
The presentation will be held in English and it will take place on Friday February 28th 2020 at 15h00 at Genopode building (UNIL), Auditorium B.
The defense will be followed by an apéro (starting around 16h00). Please fill in the following doodle to indicate your attendance:

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Group leader position available at the IGFL, Lyon (FR) – Deadline April 11, 2020

Job offer PDF

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Life Science Career Day, May 7, 2020, SwissTech convention center (EPFL)

A FREE event to get ready for your professional future!

  • Boost your career and get guidance. Learn how to communicate successfully with prospective employers, get ready for a job interview, and valorize your competences.
  • Discover positions in the corporate world, non-profit organisations & the public sector.
  • Expand your network! The networking forum is a perfect occasion to exchange insights with professionals & alumni from many organizations and companies.
  • In summary: The main goal of the LSCD is to provide guidance, tools & networking opportunities. Such information will help you to build a career after having obtained your degree or a postdoctoral experience.

 

Further information:

 

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Welcome to Albert !

My name is Albert Mestres and I am a Master student trainee in the Fajas’ lab for the spring semester 2020. I come from Barcelona where I am currently doing a Masters degree on Biomedical Metabolism and Endocrinology.

My work at the CIG is related to the action of the cell cycle regulator CDK4 in the control of metabolism. The project will be the continuation of the one done by Kelly, a previous Masters student of the lab.

 

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PhD student position available in at the TUM, Munich (DE)

PhD position Ranf lab 2-2020