J Biol Chem; group Thorens

J Biol Chem. 2010 Feb 9. [Epub ahead of print]

Glucagon-like peptide-1 (GLP-1) increases beta-cell glucose competence and proliferation by translational induction of insulin-like growth factor-1 receptor (IGF-1R) expression.

Cornu M, Modi H, Kawamori D, Kulkarni RN, Joffraud M, Thorens B.

University of Lausanne, Switzerland;

Glucagon-like peptide-1 protects beta-cells against apoptosis, increases their glucose competence and induces their proliferation. We previously demonstrated that the anti-apoptotic effect was mediated by an increase in IGF-1 receptor expression and signaling, which was dependent on autocrine secretion of IGF-2. Here, we further investigated how GLP-1 induces IGF-1R expression and whether the IGF-2/IGF-1R autocrine loop is also involved in mediating GLP-1-increase in glucose competence and proliferation. We show that GLP-1 up-regulated IGF-1R expression by a protein kinase A-dependent translational control mechanism whereas IBMX, which led to higher intracellular accumulation of cAMP than GLP-1, increased both IGF-1R transcription and translation. We then demonstrated, using MIN6 cells and primary islets, that the glucose competence of these cells was dependent on the level of IGF-1R expression and on IGF-2 secretion. We showed that GLP-1-induced primary beta-cell proliferation was suppressed by Igf-1r gene inactivation and by IGF-2 immunoneutralization or knockdown. Together our data show that regulation of beta-cell number and function by GLP-1 depends on the cAMP/protein kinase A mediated-induction of IGF-1R expression and the increased activity of an IGF-2/IGF-1R autocrine loop.

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